Fig. 2

Gossypetin decreases Aβ level in 5xFAD mice. A–E Representative image of Aβ plaques (6E10) in hippocampus and cortex of 5xFAD and gossypetin treated 5xFAD mice. Scale bar corresponds to 200μm (A). Bar graph represents quantification of Aβ plaque area (B) and number of Aβ plaque (C) in dentate gyrus of hippocampus (n = 10~12 mice per group, 3~11 slices per brain). Percentage of Aβ plaque area (D) and number of Aβ plaque (E) were measured in cortex as well (n = 10~12 mice per group, 3~10 slices per brain). F, G Level of Aβ oligomers (A11) were compared through dot blot assay (F). Bar graph represents quantification of relative level of Aβ oligomers (G). H–J Expression level of APP, Aβ and GAPDH in hippocampus were examined through Western blot analysis (H). Bar graph represents quantification of protein level of Aβ monomer (I) and APP (J) normalized by GAPDH. K–N enzyme-linked immunosorbent assay (ELISA) was conducted to quantify the level of soluble Aβ40 (K), Aβ42 (L), insoluble Aβ40 (M), and Aβ42 (N) in the hippocampus (n = 11~12 mice per group). The error bars represent the mean ± SD (G, I-N) or mean ± SEM (B-E). ***p < 0.001, **p < 0.01, *p < 0.05, ns = not significant, Student’s t test